README – Recombining Split FASTQ Files

(These fastqs are associated with figure 4d)

These FASTQ files were split into ~2 GB chunks due to Dataverse upload limits.
To reconstruct the original files after downloading all parts:

This is an example for the 6-week rat sample. Please apply the same steps
to the other samples to regenerate the full FASTQs.

Make sure all .part.* files for each FASTQ are in the same directory, then run:

# For I1
cat RA-6wk-F_S1_L004_I1_001.part.* > RA-6wk-F_S1_L004_I1_001.fastq.gz

# For I2
cat RA-6wk-F_S1_L004_I2_001.part.* > RA-6wk-F_S1_L004_I2_001.fastq.gz

# For R1
cat RA-6wk-F_S1_L004_R1_001.part.* > RA-6wk-F_S1_L004_R1_001.fastq.gz

# For R2
cat RA-6wk-F_S1_L004_R2_001.part.* > RA-6wk-F_S1_L004_R2_001.fastq.gz

After running these commands, you’ll have the original FASTQ.gz files back.
es back.

#SAMPLE PREFIX IDENTIFICATIONS


WT-EC-Rat_2Wks = 2 week old rat samples
RA-6wk-F = 6 week old rat samples

WT_CKDL210019813 - 12 week old rat samples